Diet induced obesity in zebra fish book

Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. To date, animal models of obesity based on gene modi. The prevalence of obesity is increasing worldwide, with high fat diet hfd as one of the main contributing factors. Because of this, the zebrafish has become increasingly important for the identification of genes or potential drugs regulating lipid metabolism, at accumulation and associated processes 9, 16. Basic nutritional investigation ingested cocoa can prevent. Highfat diet impairs cognitive function of zebrafish. Jun 24, 2012 backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. Fatrich diets not only induce obesity in humans but also make animals obese. Jul 07, 2017 birse rt, choi j, reardon k, rodriguez j, graham s, diop s, et al. Metabolic and bone effects of highfat diet in adult zebrafish. In zebrafish, lipids are also stored in visceral, intramuscular, and subcutaneous adipocytes, providing the opportunity to understand the regulation of body fat distribution in cases such as obesity.

Dietinduced dysbiosis of the intestinal microbiota and the. It is intended to mimic the most common cause of obesity in humans. Diet induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. It is very hardy and adaptable which makes it an ideal beginner fish. Thus, zebrafish are a useful model of human dietinduced obesity, which. The world health organization estimates that 310 million people worldwide are obese 1. Obesity is a major cause of type 2 diabetes mellitus t2dm in mammals. Most of those studies compared lean and obese animals and were based on excessive overfeeding of adult zebrafish with their natural diet in. Hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. Zeigler zebrafish diet, 1lb bag during this covid19 pandemic, pentair global operations and supply teams are working diligently to help ensure our valued customers are getting the best possible service and delivery during this time. The dio zebrafish can be used to identify putative pharmacological targets and to test novel drugs for the treatment of human obesity. Diabetes mellitus dm affects over 10% of the worlds population.

Controlled highfat diet induces an obese syndrome in rats. Dietinduced obesity in male c57bl6 mice decreases fertility. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity. Mar 21, 2017 moreover, dietinduced obesity in zebrafish shares common pathophysiological pathways with obesity in mammals 1215. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Zebrafish as a model for obesity and diabetes ncbi.

The purpose of this study was to establish a zebrafish model of dietinduced obesity dio. Highfat diet impairs cognitive function of zebrafish scientific reports. High fat plus high cholesterol diet lead to hepatic. The first diet induced obese zebrafish model dio was reported in 2010 through overfeeding with artemia saline for 8 weeks. The zebrafish danio rerio is an established model organism for the study of various metabolic diseases. Persistent impaired glucose metabolism in a zebrafish. Wistar rats are also used in studies where obesity is diet induced, and the results have shown that the body weight is increased.

Ro dent mo del for the study of obesityrelated disor ders rev assoc med bras 2012. Dietinduced obesity model of zebrafish with high fasting blood glucose it was previously reported that development of dio in zebrafish requires no less than 150 calories from freshly hatched. Zebrafish as an experimental model for the study of obesity. Research article a novel wistar rat model of obesity. Animal models of obesity are required to help us understand the signaling pathways underlying this condition. It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. Pharmacological evaluation of the mechanisms involved in. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. We have previously established a zebrafish model of diet induced obesity dio zebrafish by overfeeding artemia. The aim of this study is to examine the agerelated influence of hfd induced obesity on mandibular bone architecture and the structure of the periodontium in. Body fat accumulation in zebrafish is induced by a diet rich. Effects of yuzu citrus junos siebold ex tanaka peel on. The other group of zebrafish was fed with artemia sufficient to meet their energy requirements 5 mg dry weightdayfish.

Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with dietinduced obesity dio were evaluated. However, it is as yet unclear whether highfat diet hfd induced obesity in childhood and adolescence determines agerelated changes in jaw bone health. Dietinduced adipocyte number increase in adult rats. An investigation into the antiobesity effects of morinda. So what are we to make of our 26yearold man, who came in with neverbeforeseen levels of endotoxin producing enterobacter and walked out 23 weeks later with undetectable levels and over 100 pounds. Pdf resveratrol ameliorates dietinduced dysregulation of. Dec 22, 2017 an increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Nov 14, 2015 hf and hfc diets induced hepatic steatosis in zebrafish larvae. Related publications genetic determinants of energy expenditure and insulin resistance in dietinduced obesity in mice. Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer. In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this. Compositions of real and mimetic cocoa are listed in table 2.

Short chain fatty acids prevent highfatdietinduced obesity. Our results suggest that a diet rich in fat, instead of protein or carbohydrate, induced body fat accumulation in zebrafish with mechanisms that might be similar to those in mammals. The present study was performed to investigate the molecular mechanism of 6gingerol on adipocytemediated systemic inflammation in vitro and in highfat diet induced obese zebra fish. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. These diets were based the ain93g diet and beef tallow was added to provide a highfat content. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Can a high fat paleo diet cause obesity and diabetes. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. In 2010, oka and collaborators proposed a dietinduced obesity dio protocol by a diet administered over a short time in adult zebrafish, and in 2011, other groups proposed a test to evaluate obesity in larvae. Everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity.

These data show that hyperglycemia can be induced and maintained in zebrafish. Hyperglycemia is the main feature for the diagnosis of this disease. E2f8 promotes hepatic steatosis through fabp3 expression in. Rat models of dietinduced obesity and high fatlow dose. Highfat dietinduced obesity triggers alveolar bone loss and. The gi tract functions as a major immunological organ as it must maintain tolerance to commensal and dietary antigens while remaining responsive to pathogenic stimuli. We carried out physiological tests, plasma lipid assays, obesity measures, liver. Body fat accumulation in zebrafish is induced by a diet rich in fat. Highfat dietinduced obesity triggers alveolar bone loss.

Typically mice, rats, dogs, or nonhuman primates are used in these models. High fat diets and general overfeeding diets both show rapid increases in adipose deposition, increased bmi, hepatosteatosis, and hypertriglyceridemia. Yuzu peel significantly suppressed the rise in plasma triacylglycerol tg and liver lipid accumulation. The evidence presented shows that the zebrafish is a useful model to study obesity and its status as a risk factor for. Moreover, a useful zebrafish model of diet induced obesity dio has been created and was found to be highly consistent with the obesity observed in humans and in rodent models of dio oka et al. Diet induced obese dio mouse models c57bl6ntac male. Although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for dietinduced obesity dio, similar to that observed in mammals.

The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. In this paper, hyperglycemic zebrafish, when immersed in a 111 mm glucose solution for 14 days, developed increased glycation of proteins. The first dietinduced obese dio zebrafish model was reported in. The burchells zebra prefer open woodland, scrub and grassland. The overall gene expression profile of visceral adipose tissue is also consistent with those of humans and rodents.

Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or. Effects of yuzu citrus junos siebold ex tanaka peel on the. The aim of this study was to generate an obese adult zebrafish. Childhood obesity and the zebrafish as a model for the study of diet. Development of a novel zebrafish model for type 2 diabetes. Highfat diet induced obesity and heart dysfunction are regulated by the tor pathway in drosophila. Different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. Zebrafish, zebra fish, zebra danio scientific name. An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. To determine whether this also occurs in larvae, zebrafish fed with hf diet and hfc diet were stained with oil red o. Pdf resveratrol ameliorates dietinduced dysregulation. Dietinduced obesity in c57bl6j mice causes increased renal.

High fat plus high cholesterol diet lead to hepatic steatosis. Overfeeding and a highfat hf diet induce body fat accumulation in zebrafish through pathophysiological pathways common with those underlying mammalian obesity. Rats were examined for effects of cocoa ingestion with highfat diets. We optimized protocols for generating dietinduced obese dio zebrafish model by feeding high cholesterol diet hcd. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 % and 11 %, respectively. Short chain fatty acids prevent highfatdietinduced. Diet induced obesity zebrafish models are useful, as diet can be modified from a very early age. Oct 21, 2010 although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for diet induced obesity dio, similar to that observed in mammals. Feeding hcd for 8 weeks resulted in exogenous obesity confirmed by higher body mass index bmi values of obese males 0.

Zebra fish prefers neutral to slightly acidic water. The gastrointestinal gi microbiota is the collection of microbes which reside in the gi tract and represents the largest source of nonself antigens in the human body. Macrophages are ubiquitously present innate immune cells in humans and animals belonging to both invertebrates and vertebrates. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. They are very dependent on water and you will seldom see them more than 12 km from it. Backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. However, it is as yet unclear whether highfat diet hfdinduced obesity in childhood and adolescence determines agerelated changes in jaw bone health. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of obesity. Hf or hfc diets exposure results in steatosis and hepatitis in adult fish and rodents models 25, 35. The effect of diet and exercise on intestinal integrity and microbial diversity in mice. Obesity is a multifactorial disorder influenced by genetic and environmental factors. Because of the relatively high fat content in artemia, as compared with flake foods, we fed the zebrafish a diet consisting solely of artemia to induce obesity. The cape mountain specie prefers the mountainous areas of the eastern and western cape in southern africa.

The gut microbiota as an environmental factor that regulates fat storage. Adult zebrafish were allocated to four diet groups and over 6 weeks. Dietinduced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. Childhood obesity and the zebrafish as a model for the. Consequently, zebrafish might serve as a good animal model for.

The first dietinduced obese zebrafish model dio was reported in 2010 through overfeeding with artemia saline for 8 weeks. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity. We have characterised molecular adaptation to high fat diet hfd feeding in inbred mouse strains widely used in genetic and physiological studies. The relationship between highfat food consumption and obesity is wellestablished. Body fat accumulation in zebrafish is induced by a diet. Recent research with the gut microbiota of zebra fish support this hitchhiker theory for the movement of endotoxins from the gut to the blood. The aim of this study is to examine the agerelated influence of hfdinduced obesity on mandibular bone architecture and. Wet weight and cellularity of dissected adipose depots of adult rats fed a highfat diet for 5 mo gonadal bdy wt, retroperitmeal inguinal g total lipid, cell size, pg no. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of. Since rats and mice show a similar relationship, they are considered an appropriate model. Dietinduced dysbiosis of the intestinal microbiota and. Short chain fatty acids prevent highfat diet induced obesity in mice by regulating g proteincoupled receptors and gut microbiota. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 %. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebra.

Nutritional factors play important roles in the etiology of obesity, type 2 diabetes mellitus and their complications through genotype x environment interactions. The present study was performed to investigate the molecular mechanism of 6gingerol on adipocytemediated systemic inflammation in vitro and in highfat dietinduced obese zebra fish. Dietinduced obesity in zebrafish shares common pathophysiological pathways. This analysis revealed that obese zebrafish and mammals share common. Oct 21, 2010 obesity is a multifactorial disorder influenced by genetic and environmental factors. One group of zebrafish was overfed with artemia 60 mg dry weightday fish, a living prey consisting of a relatively high amount of fat.

Nutrigenomics of high fat diet induced obesity in mice. Zebra fish models for diabetes linkedin slideshare. These reports suggest that zebrafish can be a useful animal model for obesity research. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. We carried out physiological tests, plasma lipid assays, obesity. E2f8 promotes hepatic steatosis through fabp3 expression. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of. Pdf dietinduced obesity in zebrafish shares common. Diet induced obese dio mouse models c57bl6ntac male mice. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific. Short chain fatty acids prevent highfatdietinduced obesity in mice by regulating g proteincoupled receptors and gut microbiota. Birse rt, choi j, reardon k, rodriguez j, graham s, diop s, et al. May 20, 2015 in light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by diet induced obesity dio 15, 16, atherosclerosis induced by highcholesterol diet and in a. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd.

Backhed f, ding h, wang t, hooper lv, koh gy, nagy a, et al. Effects of glucose treatment up to 14 days on blood glucose levels. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted. We developed a rat model of diet induced obesity that will be a powerful tool for assessment of this issue. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Dietinduced obesity in c57bl6j mice causes increased. Diet induced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. In four experiments, longevans rats ate ad libitum a synthetic semipurified diet containing 20 g hf or 4 g lowfat lf of fat100 g of diet or a nonpurified diet. The zebrafish is a shoaling fish and it should never be kept in groups of less than 68 fish. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Research open access transcriptome analysis of antifatty. Highfatdietinduced obesity and heart dysfunction are regulated by the tor pathway in drosophila. Jun 19, 2007 everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity.

In this study, we used a dietinduced obesity approach in zebrafish danio. In light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by dietinduced obesity dio 15, 16, atherosclerosisinduced by highcholesterol diet and in a. Researchers now describe the first genetic model of obesity in a fish. We have demonstrated that zebrafish overfed with artemia showed significant increases in bmi and plasma tg levels and hepatosteatosis, consistent with obesity observed in humans and rodent models of dio 1, 6, 7. Moreover, a useful zebrafish model of dietinduced obesity dio has been created and was found to be highly consistent with the obesity observed in humans and in rodent models of dio oka etal. The ideal aquarium for zebra fish contains clean oxygen rich water and has a lot of open area to swim in and some areas with dense vegetation.